Part:BBa_K4162003

ybbO coding for retinol dehydrogenase, codon optimized for E. coli

Introduction

The ybbO gene in E. coli 83972 is annotated in E. coli strain MG1655 as a predicted oxidoreductase and has the highest identity and similarity (31% and 52%, respectively) in the E. coli genome to H. sapiens retinol dehydrogenase based on the BLASTP analysis of NCBI (http://www.ncbi.nlm.nih.gov/blast/).^[1] Through sequence comparisons and phylogenetic analysis that the ybbO gene may be an example of horizontal gene transfer from a eukaryotic retinol dehydrogenase ancestor.^[2]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 28
Illegal AgeI site found at 621
1000
COMPATIBLE WITH RFC[1000]

References

↑ Jang, Hui-Jeong et al. “Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system.” Microbial cell factories vol. 10 59. 29 Jul. 2011, doi:10.1186/1475-2859-10-59
↑ Baker, M E. “Evolution of mammalian 11beta- and 17beta-hydroxysteroid dehydrogenases-type 2 and retinol dehydrogenases from ancestors in Caenorhabditis elegans and evidence for horizontal transfer of a eukaryote dehydrogenase to E. coli.” The Journal of steroid biochemistry and molecular biology vol. 66,5-6 (1998): 355-63. doi:10.1016/s0960-0760(98)00064-8

[1] Jang, Hui-Jeong et al. “Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system.” Microbial cell factories vol. 10 59. 29 Jul. 2011, doi:10.1186/1475-2859-10-59

[2] Baker, M E. “Evolution of mammalian 11beta- and 17beta-hydroxysteroid dehydrogenases-type 2 and retinol dehydrogenases from ancestors in Caenorhabditis elegans and evidence for horizontal transfer of a eukaryote dehydrogenase to E. coli.” The Journal of steroid biochemistry and molecular biology vol. 66,5-6 (1998): 355-63. doi:10.1016/s0960-0760(98)00064-8

[1]

[2]